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An ancestral helical bundle governs whether the crRNA-processing nuclease of Crispr-Cas12a is exploited by a phage-encoded allosteric anti-Crispr.
The involvement of the CBD peptide in ISR was evaluated by incubating cucumber seedlings in a 25 μ m CBD solution for 48 h and subsequently infecting the cotyledons with the bacterial pathogen Psl. Intriguingly, the association of dPER(ΔCBD) with dCLK was only observed during the dark phase, unlike wild-type dPER (Fig. 5A, top, compare lanes 1 and 8).
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